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. 2013 Dec 9;3:3456. doi: 10.1038/srep03456

Figure 7. IL-17A promotes breast cancer cell migration and invasion.

Figure 7

(A) MCF7 cells were cultured in complete medium alone (medium) or supplemented with 1, 10 or 100 ng/ml of recombinant human IL-17A (IL-17A) as indicated. Cell migration was evaluated in transwell migration assay (upper panel, representative photomicrographs; lower panel, quantification: mean ± SEM of two independent experiments). (B and C) MDA-MB231 were cultured for 2 days in complete medium alone (medium) or supplemented with 100 ng/ml of recombinant human IL-17A as indicated. Cell invasiveness was then evaluated using Matrigel Invasion Chambers (B, upper panel, representative photomicrographs; lower panel, quantification: mean ± SEM of three independent experiments) and using 3D Clusters assays (C, upper left: invasive boarder of untreated cells; lower left and right: invasive boarder of IL-17A-treated cells; upper right: invasion of the surrounding ECM by IL-17A treated cells). (* P < 0.05; ** P < 0.01; *** P < 0.001).