Table 3.
Sequences of the probes immobilized on the HPV genotyping 9G membranes
| HPV genotyping 9G membrane | |||
|---|---|---|---|
| Line | Probes | Type | Sequence |
| T16 | Probe 1 | HPV16 | 5’-GGGGGGGGG TTTTTTTTT GTA CCT ACG ACA AGG GGA GG-3’ |
| T18 | Probe 2 | HPV18 | 5’-GGGGGGGGG TTTTTTTTT GTA TAG CAG ACT TGT TGA GG-3’ |
| T45 | Probe 3 | HPV45 | 5’-GGGGGGGGG TTTTTTTTT GTA TAG TAG ACA AGT GGA GG-3’ |
| T33 | Probe 4 | HPV33 | 5’-GGGGGGGGG TTTTTTTTT ATA TAT AAG ACA AGT TGA AG-3’ |
| T31 | Probe 5 | HPV31 | 5’-GGGGGGGGG TTTTTTTTT GTA TTT AAG ACA AGG TGA GG-3’ |
| HC | Probe 6 | HC | 5’-GGGGGGGGG CTTTATTTT CC ACT GTT CTC GGC ACG-3’ |
| PCR | Probe 7 | PCR | 5’-GGGGGGGGG CTTTATCTT GAC ATG KKG ARG ART ATG A-3’ |
| PC | Probe 8 | PC | 5’-GGGGGGGGG TGATTT ACA GTT TAT DTT TC-3’ |
| HPV2 genotyping 9G membrane | |||
| Line | Probes | Type | Sequence |
| Line-A | Probe 9 | HPV66 | 5’-GGGGGGGGG TTTTTTTTT ATA CCT TCG CCA AGT GGA GG-3’ |
| Probe 10 | HPV52 | 5’-GGGGGGGGG TTTTTTTTT ATA CCT TCG TCA TGG CGA GG-3’ | |
| Line-B | Probe 11 | HPV59 | 5’-GGGGGGGGG TTTTTTTTT ATA TGC CAG ACA AGT GGA GG-3’ |
| Probe 12 | HPV56 | 5’-GGGGGGGGG TTTTTTTTT GTA CCT TAG ACA AGT GGA GG-3’ | |
| Line-C | Probe 13 | HPV35 | 5’-GGGGGGGGG TTTTTTTTT ATA TTT AAG GCT TGG TGA AG-3’ |
| Probe 14 | HPV51 | 5’-GGGGGGGGG TTTTTTTTT ATA TAT TAG GCA TGG GGA AG-3’ | |
| Probe 15 | HPV58 | 5’-GGGGGGGGG TTTTTTTTT ATA TGT ACG TCA TGT TGA AG-3’ | |
| Probe 16 | HPV68 | 5’-GGGGGGGGG TTTTTTTTT ATA TAT TAG GCA TGT TGA GG-3’ | |
| Probe 17 | HPV69 | 5’-GGGGGGGGG TTTTTTTTT GTT TAT AAG GCA TGG TGA GG-3 | |
| Line-D | Probe 18 | HPV70 | 5’-GGGGGGGGG TTTTTTTTT ATA TAC TAG GCT TGT GGA GG-3’ |
| Probe19 | HPV39 | 5’-GGGGGGGGG TTTTTTTTT ATA TAC CAG GCA CGT GGA GG-3’ | |
| Line-E | Probe 20 | HPV73 | 5’-GGGGGGGGG TTTTTTTTT ATA TTT AAG ACA AGC AGA AG-3’ |
| Probe 21 | HPV53 | 5’-GGGGGGGGG TTTTTTTTT GTA TGT TAG ACT TGC AGA GG-3’ | |
| Probe 22 | HPV26 | 5’-GGGGGGGGG TTTTTTTTT ATT TAT AAG ACA TGG CGA AG-3’ | |
| Target1 ( HC-Cy5-T1) | HC-Cy5 | 3’-GGATCACCGAGATACCATTGGAGACTGCG-Cy5-5’ | |
| Forward primer | FP | 3`-GCMCAGGGWCATAAYAATGG-5’ | |
| Reverse primer | RP-Cy5 | 3`-GAAAHATAAACTGTAAATCATAYTC-Cy5-5’ | |
Note: HC—probe for the Hybridization control; PC—probe for the Primer control (Positive control); PCR—probe for the PCR control; HC-Cy5-T1—Target oligonucleotide for HC probe; GGGGGGGGG—9G for immobilization of the probes on the AMCA slides; CTT TAT TTT, TTTTTTTTT—vertical spacer groups