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. 2013 Nov 22;5(11):2881–2897. doi: 10.3390/v5112881

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© 2013 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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RSV immunohistochemical stain scoring. Seven slides with two sections each of lung lobe (at least 1 cm × 1 cm) were scored for staining of RSV antigen. Slides were assessed for number of cells per 10X field with RSV antigen staining which was then given a score according to the following scale: 0 = 0, 1 = 1–10, 2 = 11–39, 3 = 40–99, 4 =>100; slides were also assessed for the number of 10X fields with RSV antigen out of 20 fields for each slide. Both groups inoculated with HEp-2-grown RSV had higher average slide scores than control or either group inoculated with Vero-grown RSV. Nebulized HEp-2-grown RSV had significantly more fields with RSV antigen than control or Vero-grown RSV groups, p < 0.05.