Figure 1.

Sustained engraftment and expression of anti-HIV genes in the peripheral blood of patients for up to three years after transplantation. (A) Level of gene marking expressed as number of copies of vector (WPRE) per 100 blood cells analyzed over time. Unique patient identifiers are listed in the upper right hand corner of graph. Limits of quantification (stippled) and limits of detection (diagonal lines) values were determined for each amplification reaction and typically were in the range of 0.05% (500 cells/million) and 0.01% (100 cells/million), respectively. (B) Expression of shRNA against tat/rev sequences in the blood and marrow of UPN0306 at 36 months. Results expressed as copies of shRNA per 8 ng of total RNA. PBMC—peripheral blood mononuclear cells, PBGC—peripheral blood granulocytic cells, BMMC—bone marrow mononuclear cells, BMGC—bone marrow granulocytic cells. Bar height in B is mean of triplicates ± SEM.