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. 2013 Sep 22;10:292. doi: 10.1186/1743-422X-10-292

Figure 1.

Figure 1

Western blot analysis of the HBsAg expressed by wild type and mutant clones, plus empty vector as a negative control. Transfected 293 T cell supernatant samples (10 μl/sample) were loaded to each lane in SDS-PAGE. HBsAg specific monoclonal antibody H166 was used as the detection antibody at 1:1000 dilution. 1: wild type; 2: sT118M; 3: sT118M-rtM204I; 4: sT118M-rtM204V; 5: sG145K; 6: sG145K-rtM204I; 7: sG145K-rtM204V; 8: sG145R; 9: sG145R- rtM204I; 10: sG145R- rtM204V; 11: empty vector.