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. 2013 Sep 22;10:292. doi: 10.1186/1743-422X-10-292

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Copyright © 2013 Zhang et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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The supernatants from each plasmid transfection were analyzed for HBsAg using five ELISA kits. (A) Zhu Hai LivZon Diagnostic INC (LZ); (B) Beijing WanTai Biological Pharmacy Enterprise Limited Company (WT); (C) Beijing Big-GBI Biotech Limited Company (GBI); (D) Beijing BioNeovan Limited Company (BN); and (E) Shenzhen Kang Sheng Bao Bio-Technology Limited Company (KSB). With the exception of ELISA kit KSB, which recognized sT118M-rtM204I (sT118M-sI195M) with reduced avidity, all of the kits had almost invariable slope or avidity to the sT118M mutant, sT118M-rtM204I (sT118M-sI195M), and sT118M-rtM204V (sT118M-sW196S) double mutant. *, p < 0.05, compared with sT118M HBsAg.