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. 2013 Sep 22;10:292. doi: 10.1186/1743-422X-10-292

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Copyright © 2013 Zhang et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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The supernatants from each plasmid transfection were analyzed for HBsAg using five ELISA kits. (A) Zhu Hai LivZon Diagnostic INC (LZ); (B) Beijing WanTai Biological Pharmacy Enterprise Limited Company (WT); (C) Beijing Big-GBI Biotech Limited Company (GBI); (D) Beijing BioNeovan Limited Company (BN); and (E) Shenzhen Kang Sheng Bao Bio-Technology Limited Company (KSB). With the rtM204I (sI195M) mutation in the backbone of sG145K, avidity was reduced significantly in four kits. The exception was with ELISA kit GBI. rtM204V (sW196S) mutation did not cause antigenic variation of sG145K HBsAg. *, p < 0.05, compared with sG145K HBsAg.