FIG. 5.

Effect of Tepary bean protease inhibitor (TBPI) on cell invasion. NIH 3T3/v-mos fibroblasts were seeded (2 × 10⁴ cells/well) on Matrigel-coated inserts with Dulbecco's modified Eagle's medium supplemented with 1% bovine serum albumin/1.5% calf serum as a control while adding 0.027 μg protein/mL (equivalent to 250 IU/mL) of TBPI for the experimental group. After 5 days incubation, the inserts were removed and the cells were incubated 5 more days and counted with a hemocytometer. The asterisk shows a statistically significant difference with respect to wild type transformed fibroblasts (t student, P ≤ 0.05).