FIG. 6.

SDS-PAGE profile of extracellular matrix degradation assay. A: NIH 3T3/v-mos fibroblasts were seeded (1 × 10⁴ cells/well) with Dulbecco's modified Eagle's medium with 5% calf serum. After 24 h, the medium was changed and supplemented with 1% bovine serum albumin/1.5% calf serum as a control while adding 0.027 μg Protein/mL (equivalent to 250 IU/mL) of TBPI to the experimental group. When confluence was reached, extracellular matrix was extracted and analyzed by SDS-PAGE at 5% polyacrylamide gel loaded with 20 μg of protein per lane, (1) non-cultured dishes, (2) untreated cells, and (3) treated cells. B: The histogram represents the band intensities evaluated in terms of relative proportion respect to pixel number by a densitometry analyzer.