Table 1.
Tissue and temperature combinations used in constructing the reference transcriptome of P. borchgrevinki
| Treatment | Liver | Brain | White muscle | Spleen | Head kidney | Gill | Lens |
|---|---|---|---|---|---|---|---|
|
Native |
|
||||||
| Control, -1 to −1.9°C |
yes |
yes |
yes |
yes |
yes |
yes |
yes |
|
Warm Acclimation |
|
||||||
| 2 days, 4°C |
yes |
yes |
yes |
- |
yes |
yes |
- |
| 4 days, 4°C |
yes |
yes |
yes |
yes |
yes |
yes |
- |
| 7 days, 4°C |
yes |
yes |
yes |
yes |
- |
yes |
- |
| 2 months, 4°C |
yes |
yes |
- |
yes |
yes |
- |
- |
|
Acute Heat Stress |
|
||||||
| Post-CTMax | yes | yes | yes | yes | yes | - | - |
Overview of the tissues included in the reference transcriptome. Total RNA was extracted from one specimen for each tissue/treatment combination and pooled at equal concentrations. P. borchgrevinki reference transcriptome was then produced by sequencing this pool on a Roche 454 GS FLX+ Titanium sequencer.