Table 2.
Annotation results of P. borchgrevinki reference transcriptome
| Database | Contigs with hits/Total number of sequences | Percentage with hits |
|---|---|---|
|
Tiered screening1 | ||
| SwissProt (BLASTx) |
14,889 / 39,758 |
37.4% |
| UniProtKB/TrEMBL (BLASTx) |
3,062 / 24,869 |
12.3% |
| NCBI’s NR (BLASTx) |
294 / 21,513 |
1.3% |
| NCBI’s NT (BLASTn) |
5,615 / 21,219 |
26.4% |
|
Screening all sequences2 | ||
| SwissProt (BLASTx) |
14,889 / 39,758 |
37.4% |
| UniProtKB/TrEMBL (BLASTx) |
17,951 / 39,758 |
45.1% |
| NCBI’s NR (BLASTx) |
17,877 / 39,758 |
44.9% |
| NCBI’s NT (BLASTn) | 21,898 / 39,758 | 55% |
Breakdown of the number of contigs with BLAST hits at an e-value of 10–9 or lower in each of the screened databases. 1The first screening report shows the number of contigs with hits in each database out of those not previously identified. Tiered screening of databases was run in the order of most detailed annotations to least. 2The second screening report shows the number of contigs with hits in each database out of the total number of contigs in the reference transcriptome after clustering.