Table 1.
Primers used for multiplex PCR and nested PCR
| Species | Primer name | Sequences (5′ to 3′) | Amplification size (bp) | Sources |
|---|---|---|---|---|
| Forward primer for Anopheles |
ANF |
ATCACTCGGCTCATGGATCG |
|
Phuc et al. (2003) |
| An. minimus |
MAR |
GGGCGCCATGTAGTTAGAGTTG |
184 |
|
| An. harrisoni |
MCR |
GGTTGCCCACTCAATACGGGTG |
509 |
|
| Reverse primer for animal |
UNR |
GGTTGTCCTCCAATTCATGTTA |
|
Kent et al. (2005) |
| Human |
HUF |
GGCTTACTTCTCTTCATTCTCTCCT |
335 |
|
| Cow |
COF |
CATCGGCACAAATTTAGTCG |
561 |
|
| Pig |
PIF |
CCTCGCAGCCGTACATCTC |
453 |
|
| Dog |
DOF |
GGAATTGTACTATTATTCGCAACCAT |
680 |
|
|
P. falciparum |
PFF |
TTAAACTGGTTTGGGAAAACCAAATATATT |
205 |
Snounou etal. (1993) |
| |
PFR |
ACACAATGAACTCAATCATGACTACCCGTC |
|
|
|
P. vivax |
PVF |
CGCTTCTAGCTTAATCCACATAACTGATAC |
121 |
|
| PVR | ACTTCCAAGCCGAAGCAAAGAAAGTCCTTA |