Skip to main content
. 2013 Sep 24;12:109. doi: 10.1186/1476-4598-12-109

Figure 6.

Figure 6

AEG-1 regulates MMP1 expression at the transcriptional level through enhancing the binding of p65 to MMP1 promoter. (A) nuclear translocation of AEG-1 protein was revealed by transmission electron microscopy. Arrow, 18 nm gold particles conjugated to anti-AEG-1 antibody. N, nucleus. C, cytosol. Right, magnified view. Scale bar, 500 nm. (B) constructs of the MMP1 promoter region used for the lucife-rase reporter assay. (C) transactivating activity of AEG-1 on MMP1 promoter constructs in the SAS and FaDu cell lines. All data were expressed as mean ± SEM; n = 3. (D) ChIP assay showing AEG-1, p65 and CBP binding to the NF-κB binding site of the MMP1 promoter in SAS and FaDu cell lines. NMIgG served as a negative control. All data were expressed as mean ± SEM; n = 3. NS, not significant (p > 0.05); *, p < 0.05; **, p < 0.01; ***, p < 0,001.