Table 3.
Influence of enzymatic detoxification on the composition of the spruce hydrolysate
|
A. Laccase treatment (g/L) | ||||||||||
|
Enzymatic treatment |
Glucose |
Xylose |
Mannose |
Arabinose |
Galactose |
HMF |
Furfural |
Acetic acid |
Formic acid |
Total phenols |
| Lac-88 a |
19.7 ± 0.5 |
3.4 ± 0.1 |
3.7 ± 0.1 |
0.5 ± 0.1 |
0.5 ± 0.1 |
0.5 ± 0.1 |
0.5 ± 0.1 |
1.7 ± 0.2 |
0.2 ± 0.1 |
0.6 ± 0.1 |
| Lac-440 a |
20.8 ± 0.2 |
3.5 ± 0.1 |
4.0 ± 0.1 |
0.4 ± 0.1 |
0.5 ± 0.1 |
0.5 ± 0.1 |
0.5 ± 0.1 |
1.6 ± 0.1 |
0.2 ± 0.1 |
0.5 ± 0.1 |
| Lac-control b |
18.7 ± 0.9 |
3.1 ± 0.1 |
3.9 ± 0.2 |
0.4 ± 0.1 |
0.6 ± 0.1 |
0.5 ± 0.1 |
0.5 ± 0.1 |
1.7 ± 0.1 |
0.2 ± 0.1 |
1.1 ± 0.1 |
|
B. HRP treatment (g/L) | ||||||||||
|
Enzymatic treatment |
Glucose |
Xylose |
Mannose |
Arabinose |
Galactose |
HMF |
Furfural |
Acetic acid |
Formic acid |
Total phenols |
| HRP-11.8 c |
19.1 ± 0.4 |
3.2 ± 0.1 |
3.7 ± 0.1 |
0.4 ± 0.1 |
0.5 ± 0.1 |
0.5 ± 0.1 |
0.5 ± 0.1 |
1.7 ± 0.1 |
0.2 ± 0.1 |
0.9 ± 0.1 |
| HRP-59 c |
19.7 ± 0.5 |
3.3 ± 0.1 |
4.1 ± 0.1 |
0.4 ± 0.1 |
0.6 ± 0.1 |
0.5 ± 0.1 |
0.5 ± 0.1 |
1.6 ± 0.1 |
0.2 ± 0.1 |
0.9 ± 0.1 |
| HRP-control b | 18.4 ± 0.7 | 3.6 ± 0.3 | 3.7 ± 0.3 | 0.4 ± 0.1 | 0.5 ± 0.1 | 0.5 ± 0.1 | 0.5 ± 0.1 | 1.7 ± 0.1 | 0.2 ± 0.1 | 1.2 ± 0.1 |
a Lac-88 and Lac-440 represent the hydrolysates that were treated with 88 mg and 440 mg laccase, respectively.
b Lac-control and HRP-control represent the control hydrolysates for the treatments with laccase and horseradish peroxidase, respectively.
c HRP-11.8 and HRP-59 represent the hydrolysates that were treated with 11.8 mg and 59 mg horseradish peroxidase, respectively.