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Co-immunoprecipitation (co-IP) in A2780 cells treated with 0.1% DMSO or 7 μM Olaparib for 1 hr. Cells were washed twice and incubated for 30 min to remove excess inhibitor. Cells were then lysed and incubated with PARP1 antibody-protein A magnetic beads. Following washing, protein complexes were eluted from the beads, boiled and run on a gel. Western blotting was done on 0.1% XRCC5 and 1% TOP2A total lysate or on the IP protein using antibodies against XRCC5 (A) or TOP2A (B).
