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TOP2A DNA relaxation assay. TOP2A was incubated with pAcGFP1 DNA for 30 min at 37°C. The reaction was stopped using SDS and protein was digested by proteinase K. (1) 1kb DNA ladder; (2) pAcGFP1 DNA; (3) DNA incubated with TOP2A; DNA incubated with TOP2A and (4) 100 μM Etoposide; (5) 500 nM Olaparib, (6) 1 μM Olaparib; (7) 10 μM Olaparib; (8) 100 μM Olaparib.
