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. Author manuscript; available in PMC: 2014 Aug 15.
Published in final edited form as: J Am Chem Soc. 2013 Aug 1;135(32):10.1021/ja405106u. doi: 10.1021/ja405106u

Figure 3.

Figure 3

Solution-phase screening of initial hits (4th round). (a) Selective labeling of bicyclic peptides with TMR and their release from individual beads by base hydrolysis. (b) Evaluation of the 44 released bicyclic peptides for binding to TNFα in solution by fluorescent anisotropy using a fixed concentration of TNFα (5 μM) and TMR-labeled bicyclic peptide (100 nM).