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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Eur J Immunol. 2013 Aug 27;43(11):10.1002/eji.201343633. doi: 10.1002/eji.201343633

FIGURE 2. In vivo expansion of 2.5mi+ T Cells by CTB-2.5mi.

FIGURE 2

NODxB6.Foxp3EGFP F1 females (n=3) were immunized i.p. with 6.5 nmol of synthetic peptide 2.5m or CTB-2.5mi. Four days later, expansion of antigen-specific CD4 T cells was analyzed by staining with Ag7/2.5mi or Ag7/GPI282–292 control tetramers and analyzed by FACS.

(A) Representative FACS profiles showing the percentage of splenic 2.5mi+ effector T cells and 2.5mi+Foxp3+ Treg. T cells were gated on B220−, CD8−, PI− and CD4+.

(B) Bar diagram of the same experiment showing the mean ± SEM of the percentage 2.5mi+ effector T cells (top) and 2.5mi+Foxp3+ Treg (bottom; **p<0.01, ***p<0.0001). The diagram was generated from 2 independent experiments, using 3 mice per experiment and type of treatment.