Table 2.
Recovery and stability of succinyl-CoA according to sample storage condition
|
Recovery data | |||||
|---|---|---|---|---|---|
| Level of protein tested (μg) | Level of standard added (μM) | n | Recovery% | CV% | |
| 33 |
75 |
4 |
73.79 |
12.61 |
|
| 66 |
75 |
6 |
83.97 |
6.46 |
|
| 166 |
75 |
3 |
70.83 |
14.72 |
|
|
Sample storage stability | |||||
| |
n |
Mean Conc (μM) |
CV% |
CV% inter-day |
% difference |
| Fresh liver |
|
|
|
|
|
| day 1 |
5 |
60.88 |
1.72 |
|
day 1 vs day 2: 7.7 |
| day 2 |
5 |
56.20 |
0.90 |
|
day 2 vs day 3: 6.3 |
| day 3 |
5 |
52.64 |
3.25 |
|
day 1 vs day 3: 13.5 |
| |
15 |
|
|
7.30 |
|
| Nitrogen snapped Liver |
|
|
|
|
|
| day 1 |
5 |
58.72 |
5.05 |
|
day 1 vs day 2: 11.6 |
| day 2 |
5 |
51.93 |
8.21 |
|
day 2 vs day 3: 5.8 |
| day 3 |
5 |
48.94 |
1.39 |
|
day 1 vs day 3: 16.7 |
| |
15 |
|
|
9.43 |
|
| Fresh vs Nitrogen |
30 |
54.89 |
|
8.21 |
6 |
| Control standard 75 μM |
|
|
|
|
Accuracy% |
| day 1 |
2 |
60.84 |
17.99 |
|
|
| day 2 |
2 |
69.80 |
10.82 |
|
|
| day 3 |
2 |
68.70 |
1.06 |
|
|
| day 4 |
2 |
64.69 |
0.51 |
|
|
| day 5 |
2 |
65.83 |
3.23 |
|
|
| day 6 |
2 |
69.57 |
6.84 |
|
|
| 12 | 66.57 | 8.18 | 88.76 | ||
The best recovery ratio was observed with 66 μg of protein. The results of storage stability correspond to the mean concentration of SSCoA (μM) formed by 66 μg of protein over 30 min of incubation at 37°C in presence of 200 μM of AdoCbl and 400 μM of MMCoA. Two vials of a known concentration of the standard (75 μM) were included as quality control.