Table 3.
Stability of succinyl-CoA after completion of experiences and quality control test with succinyl-CoA standard solutions
|
Post-process stability | |||||
|---|---|---|---|---|---|
| Sample ID | Injection count | Time after the first injection (hours) | Mean concentration of SSCoA (μM) | CV% inter-injection | |
| Bovine-liver-1 |
2 |
33 |
33.74 |
9.22 |
|
| Bovine-liver-2 |
2 |
39 |
65.59 |
7.25 |
|
| Bovine-liver-3 |
2 |
40 |
56.11 |
6.67 |
|
| Bovine-liver-4 |
2 |
45 |
51.20 |
4.55 |
|
|
Standard (SSCoA) |
|
High Qc |
Middle Qc |
Low Qc-2 |
Low Qc-1 |
|
Nominal value |
|
500 μM |
62.5 μM |
7.8 μM |
3.9 μM |
| day 1 |
|
|
|
|
|
| |
|
485.14 |
59.99 |
7.11 |
3.53 |
| |
|
415.51 |
63.46 |
7.12 |
3.98 |
| |
|
414.23 |
51.80 |
10.20 |
4.29 |
| |
Mean (n = 3) |
438.29 |
58.42 |
8.15 |
3.93 |
| |
CV% |
9.26 |
10.25 |
21.87 |
9.67 |
| day 2 |
|
|
|
|
|
| |
|
441.52 |
67.30 |
7.86 |
3.75 |
| |
|
435.70 |
56.49 |
7.97 |
3.72 |
| |
|
433.57 |
56.71 |
7.86 |
3.51 |
| |
Mean (n = 3) |
436.93 |
60.16 |
7.90 |
3.66 |
| |
CV% |
0.94 |
10.27 |
0.83 |
3.61 |
| day 3 |
|
|
|
|
|
| |
|
401.95 |
64.41 |
8.39 |
3.52 |
| |
|
405.45 |
64.14 |
8.48 |
3.67 |
| |
|
403.39 |
62.29 |
8.53 |
3.48 |
| |
Mean (n = 3) |
403.60 |
63.61 |
8.47 |
3.56 |
| |
CV% |
0.44 |
1.82 |
0.86 |
2.79 |
| CV% inter-days (n = 9) |
6.23 |
8.08 |
11.33 |
7.20 |
|
| Accuracy% | 85.25 | 97.17 | 104.75 | 95.29 | |
The post-process stability study used 66 μg of protein over 30 min of incubation at 37°C in presence of 200 μM of AdoCbl and 400 μM of MMCoA. The quality control (Qc) study used four concentrations of the standard succinyl-CoA. The calculated accuracies varied from 85% to 104%.