FIG. 2.

A schematic illustration of the various methods used for in vitro culture of the early secondary preantral follicles, including MD, HD with nonconditioned medium, and HD-CM: the early secondary preantral follicles were first cultured in vitro for 13 days to obtain antral follicles using the various methods, followed by IVM of oocytes in the antral follicles using two different methods detailed in the Materials and Methods section. MD, microliter drop; HD, hanging drop; HD-CM, HD of ovarian cell-conditioned medium; IVM, in vitro maturation. Color images available online at www.liebertpub.com/tea