Table 3.
Development of Early Secondary Preantral Follicles (Obtained from Deer Mice Using the Mechanical Method) in HD of Nonconditioned Medium Under In Vitro Culture into Follicle Aggregates from Day 0 to 6, Followed by Further Culturing the Aggregates from Day 7 to 13 in Either MD or HD of Nonconditioned Medium
| |
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No. (%a) of oocytes at stage |
||
|---|---|---|---|---|---|---|---|
| On day 0, no. of HD (total no. of F/Oo) | Culture method | On day 6, no. of preantral FA (F/Oo) | Culture method | On day 13, no. of antral FA (F/Oo) | GV | GVBD | MII |
| 8 (40) | HD | 8 (40) | MD | 0 (0) | 0 (0) | 0 (0) | 0 (0) |
| 8 (40) | HD | 8 (40) | HD | 1 (5) | 3 (7.5) | 2 (5) | 0 (0) |
a
Out of the 40 follicular oocytes used on day 0.
HD, hanging drop; FA, follicle aggregate.