Table 4.
Development of Early Secondary Preantral Follicles (Obtained Using the Mechanical Method) Cultured In Vitro in HD-CM into Preantral and Antral Follicle Aggregates and the Effect of Methods for In-Follicle Maturation of Oocytes on the Development of Oocytes in the Aggregated Antral Follicles
| |
|
|
|
|
No. (%a) of oocytes at stage |
||
|---|---|---|---|---|---|---|---|
| On day 0, no. of F/Oo per drop (in total) | On day 6, no. of preantral FA (F/Oo) | On day 13, no. of antral FA (F/Oo) | No. of FA (F/Oo) used for IVM | Method for IVM of oocytes | GV | GVBD | MII |
| 5 (100) | 20 (100) | 13 (65) | 6 (30) | Method 1 | 13 (28.2) | 3 (6.5) | 0 (0) |
| 7 (35) | Method 2 | 18 (33.4) | 10 (18.6) | 7 (13) | |||
| 3 (36) | 12 (36) | 8 (24) | 8 (24) | Method 2 | 12 (33.3) | 5 (13.4) | 3 (8.3) |
| 1 (30) | 0 (0) | 0 (0) | – | – | – | – | – |
a
Out of the total number of follicular oocytes on day 0. For the condition of five follicles per drop, it was calculated as the product of the percentage (i.e., 65%) of the development from the preantral to antral stage and the ratio of the number of GV, GVBD, and MII oocytes to the total number of oocytes in the antral follicles used for IVM.
HD-CM, HD of ovarian cell-conditioned medium; IVM, in vitro maturation.