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. 2013 Jul 9;4(4):79. doi: 10.1186/scrt230

Figure 1.

Figure 1

Cytoskeletal morphology as a function of inhibitor treatment. Alexa Fluor 488 conjugated phalloidin (green) staining showed the actin microfilament structure of non-inhibited adipose-derived stem cells (ASCs) (A), and the disrupted cytoskeleton of ASCs cultured with 2 μM cytochalasin D (B) and 50 μM blebbistatin (C). ASC nuclei were counterstained with Hoechst (blue). Cytochalasin D inhibited actin polymerization and blebbistatin inhibited non-muscle myosin type II activity, altering cell morphology. Scale bar = 30 μm.