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Proliferation as a function of oxygen levels and cytoskeletal inhibition. To assess proliferation, DNA content (μg/well) was analyzed after 0, 14 and 21 days of differentiation. Adipose-derived stem cells (ASCs) were exposed to 20% O2 and 5% O2 conditions, and treated with cytochalasin D (checkered), blebbistatin (striped) or vehicle control (solid). Oxygen levels and cytoskeletal conditions did not significantly change DNA content over 21 days.
