. Author manuscript; available in PMC: 2015 Jan 1.

Published in final edited form as: J Virol Methods. 2013 Oct 10;195:10.1016/j.jviromet.2013.09.014. doi: 10.1016/j.jviromet.2013.09.014

Table 4. Mean fluorescence intensity (MFI) values of NY99, NY99-1nt and NY99-5nt after separate amplification with primer set WNV2387F/2530R and WNV2296F/2786R.

Mean MFI values and standard deviations of NY99 (LUA10 and LUA50), NY99-1nt (LUA75) and NY99-5nt (LUA40) are shown after separate amplification with PCR primer set WNV2387F/2530R and WNV2296F/2786R. All non-template controls were yielding a MFI value below 11 which was considered as background cut-off.

Primer set		WNV2387F/2530R (amplicon length: 143 nt) mean fluorescence intensity ± SD				WNV2296F/2786R (amplicon length: 490 nt) mean fluorescence intensity ± SD
Virus-microsphere mix^a		LUA 10	LUA 75	LUA 50	LUA 40	LUA 10	LUA 75	LUA 50	LUA 40
NY99	LUA 10	2342 ± 519	122 ± 59	26 ± NA	0	5 ± 1	6 ± 4	0	0
NY99-1nt	LUA 75	494 ± 363	4311 ± 2873	24 ± NA	0	8 ± 1	60 ± 5	0	0
NY99	LUA 50	0	0	5528 ± 114	18 ± 3	0	0	13 ± 2	9 ± 1
NY99-5nt	LUA 40	0	0	86 ± 10	3290 ± 39	0	0	5 ± 2	39 ± 4

NY99 was tagged with LUA 10 when tested with NY99-1nt (LUA 75) and with LUA50 when tested with NY99-5nt (LUA40).

Bold numbers indicate correct identification. Numbers were rounded up and represent mean values of duplicates with ± SD. NA, not applicable.