Skip to main content
. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Mol Psychiatry. 2012 Jul 31;18(8):922–929. doi: 10.1038/mp.2012.104

Figure 1. ANK3 transcription start sites and alternative splicing.

Figure 1

A) ANK3 transcription start sites (TSS) were identified by 5’ RLM-RACE with mRNA isolated from different human tissue samples, including heart, kidney and five different brain samples. Start sites are plotted relative to human ANK3 mRNA clones from the NCBI database, displayed in the lower section of the plot. Transcription start sites and putative promoters for exon1b and exon1e are within a ~250 kb region containing multiple SNPs which were associated with bipolar disorder in recent GWAS, marked in black at the top of the chart. B) Reference diagram of ANK3 transcript isoforms, and corresponding protein-coding domains. The diagram incorporates the alternative first exons from the transcription initiation sites described above, as well as alternative splice isoforms annotated in NCBI for human and mouse ANK3 loci. Differential splicing of the variable large exon (exon37) produces mRNA encoding 190, 270 or 440 kD AnkG isoforms. Fifteen Nanostring nCounter probes were designed to target distinct exons or splice junctions (arbitrary color code for ease of reference).