FIG. 1.

Isolation, culture expansion, and verification of human bone marrow mesenchymal stem cells (MSCs). (A) In passage 0 (P0), freshly isolated human MSCs appeared as individual, fibroblast-like cells. (B) During culture expansion up to P3, they showed uniform growth characteristics and still maintained a fibroblast-like morphology. (C) In fluorescence-activated cell sorting analysis, P3 cells from three donors were positive for the typical MSC antigens CD44, CD73, CD90, CD105, and CD166 and negative for the hematopoietic cell markers CD14, CD34, and CD45.