Table 2.
The Average Relative Amounts of All PNGase F-Released N-Glycans of Undifferentiated and Adipogenically Differentiated (A) Human Mesenchymal Stem Cells on Day 5 and Day 15 of Differentiation Derived from Three Different Donors
|
PNGase F-released N-glycans | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| m/z | Composition | % in MSCs | % in A (day 5) | % in A (day 15) | m/z | Composition | % in MSCs | % in A (day 5) | % in A (day 15) |
| 1171.6 | H3N2 | 0.21 | 0.30 | 0.26 | 2751.3 | S2H6N3 | 0.04 | 0.02 | 0.00 |
| 1334.6 | H5N1 | 0.16 | 0.20 | 0.11 | 2779.3 | S1H5N4F2 | 0.10 | 0.13 | 0.27 |
| 1345.6 | H3N2F1 | 0.68 | 0.70 | 0.83 | 2792.3 | S2H5N4 | 0.79 | 0.86 | 1.21 |
| 1375.6 | H4N2 | 0.05 | 0.06 | 0.04 | 2809.3 | S1H6N4F1 | 0.05 | 0.04 | 0.04 |
| 1416.7 | H3N3 | 0.07 | 0.14 | 0.21 | 2839.4 | S1H7N4 | 0.02 | 0.01 | 0.00 |
| 1538.7 | H6N1 | 0.38 | 0.34 | 0.34 | 2850.4 | S1H5N5F1 | 0.08 | 0.04 | 0.09 |
| 1579.7 | H5N2 | 0.12 | 0.11 | 0.07 | 2867.4 | H6N5F2 | 0.04 | 0.07 | 0.07 |
| 1590.8 | H3N3F1 | 0.25 | 0.54 | 0.90 | 2880.4 | S1H6N5 | 1.69 | 0.79 | 0.48 |
| 1620.8 | H4N3 | 0.08 | 0.12 | 0.12 | 2938.4 | H6N6F1 | 0.06 | 0.04 | 0.06 |
| 1661.8 | H3N4 | 0.01 | 0.02 | 0.04 | 2953.4 | S1H5N4F3 | 0.04 | 0.12 | 0.24 |
| 1742.8 | H7N1 | 0.23 | 0.13 | 0.16 | 2966.4 | S2H5N4F1 | 4.11 | 7.27 | 9.09 |
| 1783.8 | H6N2 | 0.10 | 0.03 | 0.03 | 2996.4 | S2H6N4 | 0.28 | 0.73 | 0.51 |
| 1794.9 | H4N3F1 | 0.40 | 0.29 | 0.19 | 3054.5 | S1H6N5F1 | 8.77 | 5.86 | 5.24 |
| 1824.9 | H5N3 | 0.83 | 2.00 | 0.62 | 3084.5 | S1H7N5 | 0.47 | 0.33 | 0.23 |
| 1835.9 | H3N4F1 | 0.24 | 0.71 | 1.87 | 3112.5 | N6H6F2 | 0.01 | 0.01 | 0.00 |
| 1865.9 | H4N4 | 0.04 | 0.06 | 0.11 | 3142.5 | H7N6F1 | 5.58 | 0.64 | 0.43 |
| 1940.9 | S1H5N2 | 0.13 | 0.23 | 0.14 | 3200.5 | S2H7N4 | 0.01 | 0.01 | 0.00 |
| 1946.9 | H8N1 | 0.65 | 0.40 | 0.59 | 3215.6 | H6N5F4 | 0.01 | 0.00 | 0.00 |
| 1968.9 | H4N3F2 | 0.01 | 0.02 | 0.02 | 3228.6 | S1H6N5F2 | 0.02 | 0.04 | 0.07 |
| 1981.9 | S1H4N3 | 0.03 | 0.05 | 0.07 | 3241.6 | S2H6N5 | 0.44 | 0.28 | 0.41 |
| 1999.0 | H5N3F1 | 0.16 | 0.05 | 0.05 | 3257.6 | S1H7N5F1 | 0.00 | 0.00 | 0.06 |
| 2029.0 | H6N3 | 0.05 | 0.05 | 0.03 | 3299.6 | S1H6N6F1 | 0.08 | 0.02 | 0.02 |
| 2040.0 | H4N4F1 | 0.23 | 0.22 | 0.26 | 3316.6 | H7N6F2 | 0.02 | 0.00 | 0.00 |
| 2070.0 | H5N4 | 2.12 | 3.62 | 2.52 | 3329.6 | S1H7N6 | 0.58 | 0.15 | 0.07 |
| 2081.0 | H3N5F1 | 0.02 | 0.07 | 0.19 | 3357.6 | H6N7F2 | 0.03 | 0.12 | 0.18 |
| 2145.0 | S1H6N2 | 0.08 | 0.07 | 0.08 | 3415.7 | S2H6N5F1 | 2.10 | 3.87 | 4.04 |
| 2151.0 | H9N1 | 0.45 | 0.25 | 0.30 | 3445.7 | S2H7N5 | 0.27 | 0.30 | 0.20 |
| 2156.0 | S1H4N3F1 | 0.22 | 0.27 | 0.30 | 3503.7 | S1H7N6F1 | 5.29 | 1.03 | 0.96 |
| 2186.0 | S1H5N3 | 0.89 | 2.92 | 1.47 | 3591.7 | H8N7F1 | 0.37 | 0.04 | 0.11 |
| 2214.1 | H4N4F2 | 0.05 | 0.04 | 0.06 | 3602.7 | S3H6N5 | 0.14 | 0.34 | 2.08 |
| 2227.1 | S1H4N4 | 0.02 | 0.03 | 0.06 | 3660.8 | S2H6N6F1 | 0.02 | 0.00 | 0.00 |
| 2244.1 | H5N4F1 | 16.81 | 18.42 | 12.41 | 3690.8 | S2H7N6 | 0.19 | 0.03 | 0.05 |
| 2274.1 | H6N4 | 0.56 | 0.62 | 0.31 | 3776.8 | S3H6N5F1 | 0.36 | 0.27 | 1.91 |
| 2285.1 | H4N5F1 | 0.01 | 0.02 | 0.01 | 3806.8 | S3H7N5 | 0.06 | 0.09 | 0.16 |
| 2360.1 | S1H5N3F1 | 0.09 | 0.03 | 0.07 | 3864.9 | S2H7N6F1 | 2.19 | 0.59 | 1.22 |
| 2390.1 | S1H6N3 | 0.04 | 0.08 | 0.05 | 3894.9 | S2H8N6 | 0.05 | 0.02 | 0.04 |
| 2401.1 | S1H4N4F1 | 0.09 | 0.12 | 0.17 | 3952.9 | S1H8N7F1 | 0.14 | 0.02 | 0.17 |
| 2418.2 | H5N4F2 | 0.16 | 0.34 | 0.53 | 3964.9 | S4H6N5 | 0.00 | 0.02 | 0.22 |
| 2431.2 | S1H5N4 | 2.91 | 4.33 | 5.41 | 4041.0 | H9N8F1 | 0.07 | 0.00 | 0.00 |
| 2448.2 | H6N4F1 | 0.03 | 0.04 | 0.04 | 4052.0 | S3H7N6 | 0.03 | 0.00 | 0.00 |
| 2461.2 | G1H5N4 | 0.03 | 0.05 | 0.10 | 4140.0 | S2H8N7 | 0.01 | 0.00 | 0.00 |
| 2478.2 | H7N4 | 0.01 | 0.01 | 0.00 | 4226.1 | S3H7N6F1 | 0.82 | 0.17 | 0.98 |
| 2489.2 | H5N5F1 | 0.09 | 0.14 | 0.15 | 4314.1 | S2H8N7F1 | 0.15 | 0.01 | 0.10 |
| 2519.2 | H6N5 | 2.40 | 0.74 | 0.30 | 4402.2 | S1H9N8F1 | 0.05 | 0.00 | 0.03 |
| 2592.3 | H5N4F3 | 0.24 | 0.71 | 1.45 | 4587.3 | S4H7N6F1 | 0.15 | 0.03 | 0.41 |
| 2605.3 | S1H5N4F1 | 20.36 | 30.85 | 32.31 | 4675.4 | S3H8N7F1 | 0.04 | 0.00 | 0.08 |
| 2635.3 | S1H6N4 | 0.86 | 1.62 | 1.10 | 4764.4 | S2H9N8F1 | 0.00 | 0.00 | 0.08 |
| 2693.3 | H6N5F1 | 10.52 | 3.43 | 2.10 | |||||
PNGase F, peptide-N4-(N-acetyl-β-glucosaminyl) asparagine amidase F; H, hexose; N, N-acetylhexosamine; F, deoxyhexose; S, N-acetylneuraminic acid.