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. Author manuscript; available in PMC: 2013 Dec 9.
Published in final edited form as: DNA Cell Biol. 2000 Apr;19(4):10.1089/104454900314465. doi: 10.1089/104454900314465

FIG. 6.

FIG. 6

Phosphorylation of MAPK under conditions of internalization and recycling. (A) CHO cells expressing wild type or T353A mutant receptors were exposed to ligand for various periods of time (for internalization studies) or for 30 min. (B) The cells were washed and incubated without the agonist for various periods of time in the absence or presence of 100 μ M monensin (for recycling studies). The level of phosphorylated MAPK was determined by immunoblotting using phospho-MAPK (pMAPK) antibodies as described in the text. The blots were reprobed with tubulin antibody for standardization. A representative figure from three independent experiments is presented.