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. Author manuscript; available in PMC: 2013 Dec 9.
Published in final edited form as: J Genet Genomics. 2012 May 9;39(6):10.1016/j.jgg.2012.04.004. doi: 10.1016/j.jgg.2012.04.004

Fig. 1. Schematic diagram of the strategy for constructing TALENs via Golden Gate cloning method.

Fig. 1

A: the structure of plasmid pTAL3 and pCAG-TAL3. B: the TALEN target is located in exon 12 of BMPR2 with a Hind III site in the spacer region (red). The left and right TALENs binding sequences are underlined. The pCAG-BMPR2-TAL-F was constructed using two rounds of Golden Gate cloning.