Skip to main content
. Author manuscript; available in PMC: 2013 Dec 9.
Published in final edited form as: J Genet Genomics. 2012 May 9;39(6):10.1016/j.jgg.2012.04.004. doi: 10.1016/j.jgg.2012.04.004

Fig. 2. Confirmation of BMPR2 gene disruption in HCC cells.

Fig. 2

A: the PCR strategy for identification of targeting events. As indicated, the primer pair F and R were used to amplify the 506 bp DNA fragment including the TALENs target site. B: the products from samples transfected with TALENs-expressing plasmids were resistant to Hind III digestion (lane 1), whereas non-transfected sample products were completely digested (lane 2). Untreated PCR products were loaded as a control (lane 3 and lane4). M1, 100 bp DNA ladder; M2, 1 kb DNA ladder. C: DNA sequencing results indicated a 9 bp deletion in the spacer region. The two TALEN binding sites are highlighted.