Fig. 4.

Mutation of CvpA endocytic sorting motifs disrupts vesicular localization of mCherry-CvpA. Representative micrographs of HeLa cells expressing mCherry-CvpA or mCherry-CvpA proteins where diluecine residues were substituted with dialanine in DiLeu1, Dileu2, and Dileu3, and tyrosine residues were substituted with alanine in Tyr1, Tyr2, and Tyr1/Tyr2. (Scale bar, 10 μm.) (Lower Right) Graph showing the percentage of HeLa cells exhibiting tubulovesicular, punctate, or diffuse localization of ectopically expressed mCherry-CvpA proteins (n = 100).