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. 2013 Nov 18;110(49):19938–19943. doi: 10.1073/pnas.1320171110

Fig. 2.

Fig. 2.

IPMK regulates interaction of SRF to its target gene promoters. (A) IPMK deletion does not affect Elk-1 phosphorylation. Wild-type and IPMK-deleted MEFs were deprived of serum for 12 h and stimulated with 10% FBS for 1 h. Cell lysates were prepared and analyzed for Elk-1 and ERK phosphorylation by immunoblotting. (B) The ChIP assay was used to assess SRF recruitment to the c-fos promoter SRE. Deletion of IPMK in MEFs decreased SRF recruitment to the c-fos promoter. (C) CaMKIIα-Cre; Ipmkfl/fl mice exhibited ∼60% less SRF recruitment to the c-fos promoter compared with littermate Ipmkfl/fl control. The cortical tissue was analyzed for the ChIP assay. (D) IPMK is recruited to the SRE in the c-fos promoter. GST or GST-human IPMK (GST-IPMK) was overexpressed in HEK293 cells. The ChIP assay was performed by using GST antibody. Bars represent mean ± SE (n = 3). **P < 0.01; ***P < 0.001.