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. 2013 Nov 18;110(49):19938–19943. doi: 10.1073/pnas.1320171110

Fig. 4.

Fig. 4.

Dominant-negative IPMK peptide interferes with immediate early gene expression. (A) The mRNA levels of the SRF target genes c-jun and c-fos as measured via qPCR. GST or GST-SRF-BD was transfected in HEK293 cells. Cells were deprived of serum for 12 h and stimulated with 10% FBS for 30 min. For immunoblot analysis in B, cells were stimulated with 10% FBS for 1 h. (C) The ChIP assay was used to assess SRF recruitment to the c-fos promoter SRE by using HEK293 cells overexpressing GST or GST-SRF-BD. Bars represent mean ± SE (A and C) or ± SD (B) (n = 3–5). *P < 0.05; **P < 0.01; ***P < 0.001. (D) A model depicting regulation of immediate early gene expression by IPMK. IPMK binds to SRF, enhancing its stable interaction with DNA. In the absence of IPMK, SRF dissociates from its DNA targets, at least partially. By the loss of signals from SRF and its transcriptional regulators, immediate early genes cannot be fully transcribed.