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Site-specific phosphorylation of endogenous PDE3A isoforms expressed in microsomes of human myocardium. PDE3A isoforms in microsomal and cytosolic fractions of human myocardium were immunoprecipitated using anti-PDE3A C-terminal antibody. Immunoprecipitated PDE3A isoforms were identified by immunoblotting with anti-PDE3A C-terminal antibody, and their phosphorylation was analyzed with phosphospecific antibodies as indicated.
