Fig. 3.

Effects of inhibitors on antigen presentation and cytotoxic responses. (A) Enhancement of cell-surface antigen presentation by addition of inhibitors to HeLa cells expressing infected cell protein 47 (ICP47) and HLA-B27. Indicated inhibitors were added on cultured cells immediately after transfection with an ER-targeted miniprotein that after signal sequence cleavage gives rise to an HLA-B27–specific peptide precursor with the sequence ASRHHAFSFR. Cells were incubated for 48 h, and cell-surface translocation of peptide loaded HLA-B27 was followed by flow cytometry using an MHC-specific antibody. Signal was normalized to cells not treated with an inhibitor. (B) Inhibitor dose-dependent enhancement of cell-surface presentation of GSW11 epitope by CT26. CT26 cells were incubated for 48 h with indicated concentrations of inhibitors DG002A, DG013A, or DG013B and assessed for generation and presentation of the GSW11 epitope. GSW11 presentation was detected by the GSW11-specific lacZ-inducible T-cell hybridoma CCD2Z. Error bars indicate SD for each data point calculated from three separate measurements.