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. 2013 Nov 18;110(49):E4790–E4797. doi: 10.1073/pnas.1320118110

Fig. 2.

Fig. 2.

Culture filtrate derived from VirR-deficient Mtb is hyperinflammatory in BMM. (A and B) WT BMM were treated with culture filtrate derived from WT Mtb, VirR-deficient Mtb, or VirR-complemented Mtb, in varying doses. The flow-through was used to assess background cytokine induction (dashed lines). TNF-α levels (A) and IL-12 p40 levels (B) were measured in the macrophage supernatants 24 h posttreatment. Data points represent the mean ± SD of triplicate wells from one representative experiment of three. (C and D) WT BMM were treated with culture filtrate derived from WT, VirR-deficient, or VirR-complemented Mtb. In addition, BMM were treated with a [1:1] mixture of culture filtrate derived from VirR-deficient Mtb and culture filtrate derived from either WT or VirR-complemented Mtb. TNF-α (C) and IL-12 p40 (D) levels in the macrophage supernatants were measured 24 h posttreatment. Data represent the mean ± SD of triplicate wells from one representative experiment of two. ***P < 0.001.