Fig. 1.

Anatomical location and histological characterization of metastatic CRPC samples used for phosphoproteomics. Metastatic CRPC tissues were obtained from the Rapid Autopsy Program at the University of Michigan. Sixteen samples from 12 different patients are represented and prepared as previously described for phosphoproteomics (8). Red dots indicate the approximate location of the metastatic lesions analyzed. Same-colored lines represent tissues from the same patient. Patient RA53 left lung and left femur were combined due to limiting material (yellow lines). Only tissues with greater than 350 mg and 50% tumor content were evaluated. (Scale bar, 50 μm.)