Fig. 4.

Large-scale analyses of kinase activation patterns confirm intrapatient similarity across multiple, anatomically distinct metastases. (A) Phosphokinase and phospho-RTK arrays were used to analyze metastatic lesions from five different patients from anatomically distinct metastatic lesions. (B) Unique phosphopatterns were observed for each patient, and similar patterns were observed within the same patient, as shown with like-colored circles. Each observable phospho- or total protein spot from the phosphokinase and RTK arrays were used for PCA. LN, lymph node. (C) PCA analysis of all five patients confirms intrapatient kinase expression similarity and interpatient dissimilarity. (D) Grouping metastatic lesions by similar anatomical site shows no significant grouping of samples. Each phosphokinase and phospho-RTK array are spotted in duplicate, and positive control spots are located in the top left, right, and bottom left of each array. The first three principal components represent 77% of the total variance. Adrenal, adrenal gland lesions; LN, distant lymph node lesions; marrow, bone marrow lesion.