Figure 1.

TWIK-1 gene knockout mice. (A) Schematic illustration of the targeted exon 2 deletion in TWIK-1 gene. Exon 2 encodes the two ion conducting pores (P1, P2), and the transmembrane domain 2 and 3 (M2, M3) (all in shadow). (B) RT-PCR amplification targeted on the axon 2 of TWIK-1 gene was performed with the mRNAs extracted from hippocampus of WT, heterozygote (TWIK-1^+/−) and TWIK-1^−/− mice, that yielded the anticipated products as indicated. (C) Sequencing analysis from the PCR products from (B) confirmed an anticipated deletion of exon 2 in TWIK-1^−/− mouse, which encodes 132 amino acids in the position 119–250 of TWIK-1 channel.