Figure 4. Cell cycle analysis of glucose- and glyburide- stimulated rat islets.

Islets (200) were cultured for 4 days in CMRL as indicated. The medium was changed after 2 days. At the end of the 4 day period, islets were dispersed into single cells, stained with propidium iodide and processed for flow cytometry to determine cell cycle progression. Data represents the % of gated cells in each cell cycle phase. Data are representative of n = 3 experiments. Reprinted with permission from ref. [56]