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. Author manuscript; available in PMC: 2013 Dec 9.
Published in final edited form as: Open Endocrinol J. 2010;4:10.2174/1874216501004010040. doi: 10.2174/1874216501004010040

Figure 6. Effect of GSK-3 inhibition on mTOR-dependent regenerative processes in human islets.

Figure 6

Effect of LiCl (A) and 1-Akp (B) on mTOR-dependent DNA synthesis. Islets (100) were cultured for 4 days in CMRL as indicated and 3H-thymidine incorporation determined during the final 24 h. Data are the means ± S.E. of n = 2–5 experiments; *p<0.05, **p<0.01, and ***p<0.001 indicate significant differences from 8 mM glucose alone. (C) Islets (200) were cultured in CMRL as indicated. At the end of the 4-day incubation period, islets were dispersed into single cells, stained with propidium iodide and processed for flow cytometry to determine cell cycle progression. Data are the means ± S.E. of n = 3 experiments. **p<0.01 and ***p<0.001 denote significant differences between the bracketed conditions. Reprinted with permission from ref. [57].