Table 2.

Literature summary of FFA titers, yields, and percent of maximum TY^a

Base strain	Modifications	Thioesterase	Titer (g/l)	Mediac	Yieldc (% w/w)	% TY	Time (h)	Culture type	Refs
BL21(DE3)	ΔfadD, ACC+	TesA′ + CcTE	0.38	LB/none	N/A	N/A	> 18	Batch	[46]
BL21(DE3)	ΔfadD, ACC+	TesA′ + CcTE	2.5	M9/gly	4.8	12.8	22	Fed-batch	[46]
BL21(DE3)	ΔfadD, ACC+	TesA′ + CcTE	0.94	LB/None	N/A	N/A	> 20	Batch	[49]
DH1	ΔfadD	TesA′	0.7b	M9/2% glu	3.5	10.3	N/A	Batch	[14]
DH1	ΔfadE	TesA′	1.2b	M9/2% glu	6	17.6	N/A	Batch	[14]
K-12 MG1655	ΔfadD, ACC+	BTE	0.81	LB/0.4% gly	< 16.1	< 42	29	Batch	[26]
K-12 MG1655	ΔfadD	BTE	0.77	LB/0.4% gly	< 15.3	< 40	24	Batch	[55]
K-12 MG1655	ΔfadD ΔfadE ΔfadAB	BTE	0.22	MOPSc/0.4% glu	5.5	15.8	24	Batch	[52]
K-12 MG1655	ACC+, FabD+	SpTE	0.16	LB/none	N/A	N/A	24	Batch	[76]
K-12 MG1655	ΔfadD	RcTE	2.1	LB/1.5% glu	< 14	< 41	36	Batch	[62,77]
K-12 MG1655	ΔfadD	JcTE	1.5	LB/1.5% glu	< 10	< 29	36	Batch	[78]
BL21(DE3)	ΔfadE	TesA′ + CcTE	0.45	LB?	N/A	N/A	> 18	Batch	[38]
BL21(DE3)	ΔfadE, FabZ+, FabG+, FabI+	TesA′ + CcTE	0.65	LB?	N/A	N/A	> 18	Batch	[38]
K-12 MG1655	ΔfadD, SaFabD+	RcTE	1.4	LB/1.5% glu	< 9.3	< 27	24	Batch	[77]
BL21(DE3)		TesA′	5.1b	M9/glu	4.1	12.9	38	Fed-batch	[79]
BL21(DE3)	ΔfadL	TesA′	4.8b	M9/glu	4.4	12.1	38	Fed-batch	[79]
K-12 MG1655	See text, FadAB+	TesA′	~0.45b	Minimal/2% glu	2.3/7.4d	6.6/22d	48	Batch	[63]
K-12 MG1655	See text, FadAB+	TesB	~0.70b	Minimal/2% glu	3.5/13.3d	10/39d	48	Batch	[63]
K-12 MG1655	See text, FadAB+	FadM	~0.87b	Minimal/2% glu	4.4/28d	13/85d	48	Batch	[63]
K-12 MG1655	See text, FadAB+	FadM	~7	Minimal/3% glu	23/28d	70/85d	60	Batch bioreactor	[63]
DH1	ΔfadE	TesA′	3.8	Minimal/2% glu	19	56%	72	Batch	[54]

^aAbbreviations: TesA′, cytosolic form of E. coli thioesterase I; CcTE, acyl-ACP TE from Cinnamomum camphorum; BTE, acyl-ACP TE from Umbellularia californica; SpTE, oleoyl-ACP TE from Streptococcus pyogenes; RcTE, acyl-ACP TE from Ricinus communis; JcTE, acyl-ACP TE from Jatropha curcus; SaFabD, FabD from Streptomyces avermitilis; FadM, E. coli acyl-CoA thioesterase; TesB, E. coli thioesterase II (acyl-CoA thioesterase); glu, glucose; gly, glycerol; LB, Luria Bertani Broth; N/A, either not applicable because it cannot be calculated from information given, or not available because information was not provided. See references for further details.

^bFFA or extracellular fatty acids only.

^cSeveral groups performed experiments in rich media such as LB. Because LB contains other usable carbon sources (e.g., amino acids), yields from supplemented carbon (glucose, glycerol) are probably lower than the maxima reported (indicated with <).

^dAuthors’ calculation per g carbon source consumed (other values are per g carbon source supplied).