Table 1.
Effect of solution pH and NaCl concentration on thermal onset (Tonset) and thermal melting (Tm1, Tm2, Tm3) temperatures of an IgG1 mAb as determined by DSC. See methods section for experimental details. SD is standard deviation from triplicate (n=3) experiments. ND indicates a Tm value could not be determined from experimental data.
| pH | [NaCl] (M) | Tonset | SD | Tm1 | SD | Tm2 | SD | Tm3 | SD |
|---|---|---|---|---|---|---|---|---|---|
| 4.0 | 0.00 | 48.1 | 0.9 | 56.1 | 0.1 | 70.7 | 0.2 | 72.6 | 0.1 |
| 0.15 | 46.6 | 0.4 | 53.0 | 0.1 | 68.3 | 0.1 | 70.8 | 0.1 | |
| 1.00 | 44.7 | 0.2 | 50.9 | 0.1 | 67.3 | 0.1 | 68.5 | 0.1 | |
| 6.0 | 0.00 | 66.1 | 0.1 | ND | ND | 73.0 | <0.1 | 83.4 | 0.3 |
| 0.15 | 65.4 | 0.2 | ND | ND | 72.7 | <0.1 | 83.3 | <0.1 | |
| 1.00 | 62.6 | 0.5 | 69.9 | 0.1 | 74.3 | <0.1 | 84.1 | <0.1 | |
| 8.0 | 0.00 | 66.0 | 0.2 | ND | ND | 72.3 | <0.1 | 82.7 | <0.1 |
| 0.15 | 66.0 | 0.3 | ND | ND | 71.9 | <0.1 | 82.7 | <0.1 | |
| 1.00 | 64.5 | 0.1 | 71.0 | <0.1 | 73.6 | <0.1 | 83.6 | <0.1 |