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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: J Microbiol Methods. 2013 Oct 8;95(3):10.1016/j.mimet.2013.09.019. doi: 10.1016/j.mimet.2013.09.019

Figure 1. Introduction of tetO upstream of cagUT.

Figure 1

A. The genetic organization of cagUT and flanking genes is depicted. Filled arrows represent individual open reading frames. H. pylori cagU and cagT are co-transcribed as part of an operon illustrated by the open arrow (Sharma et al. 2010; Ta et al. 2012). B. Three copies of tetO were introduced into the predicted promoter region upstream of cagU: upstream from the predicted -35 region, between the predicted -35 and -10 regions, and downstream from the identified transcription start site (Sharma et al. Nature 2010). The DNA sequence of the modified promoter region is shown in Figure S1.