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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: J Microbiol Methods. 2013 Oct 8;95(3):10.1016/j.mimet.2013.09.019. doi: 10.1016/j.mimet.2013.09.019

Table 1.

Bacterial Strains and Plasmids

Strain Designation Description Reference
26695 Sequenced reference strain (Tomb, White et al. 1997)
VM120 26695 containing 3 copies of tetO inserted in cagUT promoter element, KmR
VM122 VM120 containing tetR expressed from the ureA locus, KmR CmR
VM124 26695 containing tetR expressed from the ureA locus, KmR CmR
Plasmid Designation
pAD1 Vector for introducing recombinant DNA into the H. pylori ureA locus (Ando, Israel et al. 1999; Forsyth and Cover 2000; Loh, Forsyth et al. 2004; Shaffer, Gaddy et al. 2011)
pMM680 pUC57 containing the H. pylori 26695 cagU-cagV intergenic region and introduced copies of a kanamycin resistance determinant and tetO
pMM682 H. pylori codon-optimized tetR cloned into pAD1