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. Author manuscript; available in PMC: 2013 Dec 9.
Published in final edited form as: J Androl. 2010 Apr 8;32(1):10.2164/jandrol.109.009456. doi: 10.2164/jandrol.109.009456

Figure 2.

Figure 2

Determination of the Tekt5 cDNA sequence. (A): TEKT5 peptide sequences determined by mass spectrometry (Table 2) were used to search the NCBI nucleotide database using the blastP algorithm. The four corresponding nucleotide sequences are listed, schematically showing the comparison of four possible nucleotide sequences. The same color denotes the same DNA sequence. The arrows indicate the approximate positions of primers used for cloning the testicular Tekt5 cDNA. The primer sequences are listed in Table 1. (B): Primers derived from peptide sequence of TEKT5 successfully amplified the expected size product and the amplicons were sequenced. (C): Northern blot, using the previous amplicon as probe, showed the size of Tekt5 in mixed germ cell (MGC) and round spermatid (RS) mRNA is around 2.3 kb. AKAP4 was used as a loading control.