FIG. 4.
Glycosaminoglycan (GAG) content (A) or normalized GAG content (B) at 7, 14, and 21 days of culture in (O1) osteogenically precultured hMSCs seeded at 1.5×105 cells per scaffold, (O2) osteogenically precultured hMSCs seeded at 3.0×105 cells per scaffold, (OH) 1:1 ratio of osteogenically precultured hMSCs and HUVECS seeded at 3.0×105 cells per scaffold, (OMH) 1:1:1 ratio of osteogenically precultured hMSCs, hMSCs, and HUVECs seeded at 3.0×105 cells per scaffold, (M1) hMSCs seeded at 1.5×105 cells per scaffold, (M2) hMSCs seeded at 3.0×105 cells per scaffold, (MH) 1:1 ratio of hMSCs and HUVECS seeded at 3.0×105 cells per scaffold, and (H) HUVECs seeded at 1.5×105 cells per scaffold. The results are presented as mean±SD for n=4. #, *Denote significant difference of cocultures from the corresponding monocultures (with lower and higher densities, respectively),+indicates significant difference of precultured cocultures (OH, OMH) versus coculture of non-precultured cells (MH),×denotes significant difference between monocultures with lower and higher seeding density. ‡Reflects significant difference to the previous time point for all groups (p<0.05). Color images available online at www.liebertpub.com/tea