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. Author manuscript; available in PMC: 2014 Oct 23.
Published in final edited form as: ACS Appl Mater Interfaces. 2013 Oct 8;5(20):10.1021/am401981g. doi: 10.1021/am401981g

Figure 4. Layer-by-layer assembly of functionalized hyperbranched alginate and PAMAM polymers on planar substrates.

Figure 4

(A) Selectivity and stability of azide-functionalized Si wafer illustrated via incubation of silicone azide surface with mPEG-N3 (control), followed by incubation with mPEG-MDT (bioorthogonal conjugation). Wafer was subsequently washed with PBS (Wash 1–3), followed by 4 M NaCl (Wash 4–6). (B) Ellipsometry film thickness measurements on planar azide-functionalized Si surfaces after deposition of 12 alternating polymeric layers of either: (1) PAMAM 30/0 with hyperbranched Alg-N3; (2) PAMAM 15/0 with hyperbranched Alg-N3; and (3) PAMAM 15/40 with hyperbranched Alg-N3. Controls consisted of (4) PAMAM 0/0 with hyperbranched alginate, (5) PAMAM 15/40 with hyperbranched alginate, and (6) PAMAM 15/40 with Alg-N3 (not hyperbranched). Error = standard deviation. (C) ATR-FT-IR spectra of the deposited films (2) (solid line) and (3) (dashed line). (D) Elemental composition of surfaces of Si wafers by X-ray photoelectron spectroscopy.